Polymerase chain reaction (PCR) detection of Ornthobacterium rhinotracheale (ORT)

Ciência Rural

Canal, Cláudio Wageck²  Oliveira, Silvia Dias de²  Beltrão, Nilzane²  Leão, Joice Aparecida²  Rocha, Silvio Luís da Silveira²  Fallavena, Luiz Cezar Bello¹ 

[1] Universidade Luterana do Brasil;Universidade Federal do Rio Grande do Sul, Porto Alegre

关键词: /font> Ornithobacterium rhinotracheale;    avian pathology;    PCR;    detection;    identification;   

DOI  :  10.1590/S0103-84782003000200032

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Ciencias Rurais

【 摘 要 】

Ornithobacterium rhinotracheale (ORT) is a recently discovered Gram negative bacterium that has been associated with respiratory diseases in commercial poultry and wild birds from many countries. In Brazil, antibodies were detected in some broiler and breeder flocks from the States of São Paulo and Minas Gerais. Because the bacteria is difficult to grow, the Polymerase Chain Reaction (PCR) has been found to be suitable for identification and diagnostic purposes. The aim of the present work was to verify the occurrence of ORT in Rio Grande do Sul through the detection of the bacteria DNA. Tracheal swabs (84) were collected from 14 broiler flocks of distinct companies. DNA was purified and PCR performed with species specific primers from the ORT 16S ribosomal RNA gene. Amplification products with 784 base pairs were obtained from 10 out of the 84 samples. The positive samples were from four flocks of tree companies established in different regions of the state. The results indicate that this respiratory pathogen occurs in major broiler producing areas from the State of Rio Grande do Sul. Further studies are under way to determine the prevalence of this pathogen and to characterize the strains isolated.

【 授权许可】

CC BY-NC