cDNA Cloning and Chromosomal Mapping of Rat Smad2 and Smad4 and Their Expression in Cultured Rat Articular Chondrocytes

Endocrine Journal

SHIN-ICHI SONTA²  MAKOTO OSAKI³  HIROYUKI SHINDO³  YOUICHI MIYAZAKI³  NORIO ONO²  YASUHIRO HIROTA³  AKIHIKO YONEKURA³  SHUNICHI YAMASHITA¹  TOMOO TSUKAZAKI¹ 

[1] Department of Nature Medicine, Atomic Bomb Disease Institute, Nagasaki University School of Medicine;Department of Genetics, Institute for Developmental Research, Aichi Human Service Center;Department of Orthopaedic Surgery, Nagasaki University School of Medicine

关键词: Smad2;    Smad4;    Rat;    Mapping;    Chondrocyte;   

DOI  :  10.1507/endocrj.46.695

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(23)Cited-By(2)Smad proteins are known to transduce signalling of TGF-β receptor superfamily. We report here the entire sequences of rat Smad2 and Smad4 which have not been identified yet. Entire sequences were identified by degenerated polymerase chain reaction and following phage library screening and 5' RACE. The predicted amino acid sequences of rat Smad2 and Smad4 are highly conserved among rat, human and mouse. We also mapped these Smads to chromosome 18q.12.3. Unlike endothelial cells, TGF-β1 stimulates articular chondrocyte proliferation as well as extracellular matrix production, and acts as a repairing agent against cartilage destruction. Since both Smad2 and Smad4 are essential factors for TGF-β signalling, we examined their expression and regulation in cultured articular chondrocytes. Northern blot analysis showed that TGF-β1 significantly increased the mRNA level of Smad2 but not of Smad4 in a dose- and time-dependent manner, suggesting that the augmentation of TGF-β1 action is caused by increasing the expression of the downstream signalling molecule.

【 授权许可】

Unknown