Phenotypic Characterization and Random Amplified Polymorphic DNA (RAPD) analysis of Pasteurella multocida isolated from Korean Pigs

Journal of Veterinary Medical Science

Myoung-Heon LEE⁴  Ki-Eun LEE⁴  Young-Hee OH²  Hwan-Won CHOI³  Kyung-Soo CHANG¹  Dong-Jun AN⁴  Hye-Young JEOUNG⁴  Ji-Youn LEE⁴ 

[1] Catholic University of Pusan, Busan, 609-757, South Korea;Seoul Metropolitan Government Research Institute of Public Health and Environment, Gwacheon, Gyeonggi-do, 427-070, South Korea;ChoongAng Vaccine Laboratories, Daejon, 305-348, South Korea;Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Gyeonggi-do, 430-824, South Korea

关键词: antimicrobial;    Pasteurella multocida;    RAPD;    serogroup;    swine;   

DOI  :  10.1292/jvms.11-0418

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(18)Cited-By(1)Pasteurella multocida causes various respiratory disease symptoms in pigs, including atrophic rhinitis and pneumonia. In the present study, 69 strains of P. multocida were isolated from 443 pigs with respiratory clinical symptoms at 182 farms located throughout South Korea from 2009 to 2010. A multiplex capsular PCR typing assay revealed that 69 strains of P. multocida isolated in this study had the biosynthetic locus of the capsules of either serogroup A (47 strains, 68.1%) or serogroup D (22 strains, 31.9%). The 22 strains positive for serogroup D-specific primers were divided into four clusters and the 47 strains positive for serogroup A-specific primers were divided into 12 clusters according to the results of Random Amplified Polymorphic DNA (RAPD) analysis. P. multocida strains in the present study were susceptible to most of the antimicrobial agents used. An analysis of antimicrobial resistance and virulence gene pattern combined with RAPD indicated that a certain P. multocida strain appeared to be genetically identical, implying the persistence of the strain within a single farm.

【 授权许可】

Unknown