Paternity Determination of Captive Bottlenose Dolphins (Tursiops truncatus) Using Microsatellite DNA Analysis

Journal of Veterinary Medical Science

Fumio TERASAWA²  Yukiko HORI²  Hidetoshi INOKO³  Koichi MURATA¹  Shouichi KITAMURA²  Daisuke SUMIYAMA³  Jerzy K. KULSKI³ 

[1] Department of Wild Animal Science, Nihon University;Shin Enoshima Aquarium;Department of Molecular Life Science, Tokai University, School of Medicine

关键词: bottlenose dolphin;    microsatellite DNA analysis;    paternity determination;   

DOI  :  10.1292/jvms.70.711

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(12)We applied previously published PCR primer pairs to amplify alleles at three polymorphic microsatellite loci to determine the genetic relationship of 6 bottlenose dolphins (Tursiops truncatus) that were living together in a Japanese aquarium. The three microsatellite loci were sufficient to determine the haplotype relationships of the six dolphins, which represented three different generations. It was confirmed that this genotyping method is simple and economical for assessing, establishing and maintaining genetic diversity in captive populations and will become a very effective technique for ex situ conservation in aquariums and zoos.

【 授权许可】

Unknown