Sex Determination Based on Fecal DNA Analysis of the Amelogenin Gene in Sika Deer (Cervus nippon)

Journal of Veterinary Medical Science

Koji MIYAZAKI¹  Yuji MORI¹  Shin-ichiro HAMASAKI²  Yukari TAKEUCHI¹  Kiyoshi YAMAUCHI¹  Takefumi KIKUSUI¹ 

[1] Laboratory of Veterinary Ethology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan;Wildlife Management Office, 5-8 Fuda Tama-ku, Kawasaki-city, Kanagawa 214-0011, Japan

关键词: amelogenin;    feces;    sex determination;   

DOI  :  10.1292/jvms.62.669

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(7)Cited-By(24)A sex determination method using DNA extracted from feces has been developed for sika deer (Cervus nippon). We determined a partial sequence of the amelogenin gene of sika deer, which exists on both X and Y chromosomes with a deletion region on the Y chromosome. Based on the sexually dimorphic sequences, we designed a pair of primers which could amplify DNA fragments the lengths of which are different between males and females. PCR products were detected in 34 out of 37 fecal samples collected from captured deer and the sexes estimated by the present method were perfectly matched with the actual sexes.

【 授权许可】

Unknown