Screening of Lactic Acid Bacteria for Antifungal Activity against Aspergillus oryzae | Science Publications

American Journal of Applied Sciences

Zaiton Hassan¹  Belal J. Muhialdin¹ 

关键词: A. oryzae;    antifungal activity;    API 50CHL;    Lactic Acid Bacteria (LAB);    global concern;    antifungal activity;    isolates inhibited;    spoilage moulds;   

DOI  :  10.3844/ajassp.2011.447.451

学科分类：自然科学（综合）

来源: Science Publications

【 摘 要 】

Problem statement: The growth of spoilage fungi have been a global concern because of the economy loses and the health hazard of the mycotoxins produced by the spoilage fungi. Approach: A total of 137 lactic acid bacteria isolated from Malaysian fruits and fermented foods were screened for antifungal activity using dual agar overlay method and well method against A. oryzae. Results: 23 isolates showed inhibition activity after 72 h incubation at 30°C. Supernatant of three isolates with strong antifungal activity was evaluated by well method and they inhibited the growth of the fungi at 30°C for 72 h. LAB supernatant reduced the mass growth of Aspergillus oryzae when incubated for 7 days at 30°C. The isolates were identified using API 50CH as Lactobacillus brevis G004, Lactobacillus fermentum Te007 and Pediococcus pentosaceus Te010. Conclusion: The three isolates studied inhibited the growth of the mycelia and conidia germination of the fungi which indicate the possibility of using LAB isolates as biopreservative.

【 授权许可】

Unknown