A Field study to Estimate the Prevalence of Bovine African Trypanosomosis in Butaleja District, Uganda

Journal of Veterinary Medical Science

Charles P. OTIM²  Oriel M. M. THEKISOE¹  Zhang JING¹  Noboru INOUE¹  Chihiro SUGIMOTO³  Joseph W. MAGONA³  Tatsuya SAKURAI¹ 

[1] National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine;National Livestock Resources Research Institute (NaLIRRI);Research Center for Zoonosis Control, Hokkaido University

关键词: bovine;    prevalence;    trypanosome;    Uganda;    zoonotic parasite;   

DOI  :  10.1292/jvms.71.525

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(19)Cited-By(2)Prevalence of bovine trypanosomosis was determined from a total of 203 blood samples collected from Butaleja district, eastern Uganda. All samples were examined by microhematocrit centrifuge test (MHC), PCR and ELISA. ELISA was performed in accordance with the OIE standard procedures using Trypanosoma brucei gambiense procyclic form crude antigens. PCR were utilized to identify the species and the subspecies of trypanosome. The overall prevalence of bovine African trypanosomosis was 8.9% by MHC, and 45.3% by the ELISA. Since substantial number (12 out of 18) of MHC positive samples were negative in the PCR tests, we could not conclude the most epidemic trypanosome species in the studied area. Nevertheless, the PCR results suggests that the most prevalent trypanosome was T. b. brucei (31/203), followed by T. congolense (6/203). In addition, only a few (3/203) mixed infections of T. b. brucei and T. congolense was detected by the PCR. Results obtained from this study indicates that bovine trypanosomosis is endemic in Butaleja district, Uganda.

【 授权许可】

Unknown