Mineral phase in shell repair of Manila clam Venerupis philippinarum affected by brown ring disease

Diseases of Aquatic Organisms

Christine Paillard¹  Nolwenn Trinkler¹  Aurélie Jolivet¹  Jean-François Bardeau¹  Philippe Crassous¹  Frédéric Marin¹  Maylis Labonne¹ 

关键词: Aragonite;    Hemocytes;    Shell microstructure;    Calcium carbonate;    Biomineralization;    Mollusc;    Raman microspectrometry;    Scanning electron microscopy;   

DOI  :  10.3354/dao02288

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: The mineral phase of shell repair in the Manila clam Venerupis philippinarum affected by brown ring disease (BRD) was characterised at various scales and at various stages of shell repair by confocal Raman microspectrometry and scanning electron microscopy. Spherulitic and quadrangular aragonite microstructures associated with polyene pigments were clearly observed. Von Kossa staining showed that at the beginning of shell repair, hemocytes are filled with insoluble calcium carbonate salts in all fluids and then are transported toward the extrapallial fluids and the repair sites. Our analyses suggest that after a Vibrio tapetis attack and BRD deposit some clams rapidly cover the deposit, resulting in a modification in the microstructure, which could be produced by the participation of both the mantle and hemocytes.

【 授权许可】

Unknown