Genetic diversity of Phytophthora spp. from Venezuelan cacao plantations by ISSR markers

Revista de Protecci贸n Vegetal

Pérez-Martínez, Simón¹  Isturiz Zapata, María Alejandra²  Demey, Jhonny²  Sosa, Daynet³  Molina, Sandy² 

[1] PROMETEO-SENESCYT Universidad Estatal de Milagro (UNEMI), Milagros, Ecuador;Fundación Instituto de Estudios Avanzados (IDEA), Baruta, Venezuela;Centro de Investigaciones Biotecnológicas del Ecuador (CIBE), Ecuador

关键词: cacao;    ISSR;    diversity;    Phytophthora;   

DOI  :  

学科分类：农业科学（综合）

来源: Centro Nacional de Sanidad Agropecuaria

【 摘 要 】

Phytophthora spp. comprises four species responsible for canker of the trunk and black pod of cacao (Theobroma cacao L.) in Venezuela. Pytophthora palmivora (E.J. Butler) E.J. Butler is the most widely distributed species, followed by Pytophthora megasperma Drechsler, Pytophthora capsici Leonian, and Pytophthora citrophthora (R.E. Sm. & E.H. Sm.) Leonian. In order to understand the genetic basis of the population of Phytophthora spp. on cacao, its diversity was analyzed by ISSR molecular markers. Samples from the three main cocoa growing areas of Venezuela were collected. Forty nine isolates were morphologically identified at the genus level, from which genetic profiles were generated with three out of six, ISSR primers (880, 888 and 890). A matrix 49x55 was analyzed by Principal Coordinates Analysis (ACoP), Cluster Analysis and External Logistics Biplot (BLE). The ISSR primers allowed the construction of the genetic fingerprinting of the isolates; it showed no association with the site of origin. The ACoP distinguished three main groups, which could be predictive of at least three species, which in turn would be the most frequently affecting cocoa plantations in the country. At the same time, BLE enabled us to identify that, of the total of 55 amplified fragments, only three bands of the primer 888 (1.400pb, 3.200pb, and 4.600pb) were directly involved in the separation of the groups. Thus, BLE showed not only the relationship among isolates, but at the same time the relationship between isolate-allele and allele-allele.

【 授权许可】

CC BY-NC