Arterial diffuse intimal thickening associated with enzootic calcinosis of sheep

Pesquisa Veterinária Brasileira

Barros, Severo Sales de¹  Irigoyen, Luiz Francisco¹  Grando, Silvia Maria¹  Russowski, Denise¹  Vasconcelos, Rosemeri Oliveira² 

[1] Universidade Federal de Santa Maria, Santa Maria;Universidade Estadual Paulista, Araçatuba

关键词: Enzootic calcinosis;    Nierembergia veitchii;    arterial lesions;    sheep.;   

DOI  :  10.1590/S0100-736X1998000100002

来源: Colegio Brasileiro de Patologia Animal-CBPA

【 摘 要 】

.- Vasconcelos R.O., Barros S.S., Russowski D., Grando S.M. & Irigoyen L.F. 1998. Arterial diffuse intimal thickening associated with enzootic calcinosis of sheep. Pesquisa Veterinária Brasileira 18(1):9-15. Depto Patologia, Universidade Federal de Santa Maria, 97119-900 Santa Maria, RS, Brazil.Morphometric, immunohistochemical and ultrastructural studies were carried out on the diffuse intimal thickening (DIT) in arteries of 7 sheep with clinical signs of naturally occurring enzootic calcinosis due to ingestion of the plant Nierembergia veitchii. Arterial lesions consisted of medial deposition of calcium salts and DIT. Calcification of the intima was rare, mild and located near the elastic lamina. By immunohistochemistry a-actin was detected in cells of the media and in cells forming the intimal thickening. Receptors for 1,25(OH)2 vitamin D3 were detected in nuclei of intimal, medial and endothelial cells. DIT was irregularly distributed and was neither proportionally related to the intensity of the underlying mineralization area nor to the thickening of the remaining media. Ultrastructural morphometry in smooth muscle cells (SMCs) of the media and thickened intima revealed, in the latter, an increase of 318% in the volumetric fraction of those organelles involved in synthesis and a proportional decrease in contractile elements when compared to normal values of media cells. There were histological and ultrastructural evidences of modification of SMCs and their migration to the intima, where they proliferated causing DIT. It was concluded that DIT is a consistent component of arteriosclerotic lesions in N. veitchii induced calcinosis of sheep and that the predominant cell in this process is the SMCs originated from its predecessors of the media. It is suggested that the inducing factor for the arterial changes is 1,25(OH)2 D3 present in N. veitchii.INDEX

【 授权许可】

CC BY-NC