Artificial Intravaginal Insemination Using Fresh Semen in Cats

Journal of Veterinary Medical Science

Tatsuya HORI¹  Akihiro TANAKA¹  Youko FUJIMOTO¹  Yuuko TAKAGI¹  Toshihiko TSUTSUI¹  Kiyoshi NAKAGAWA¹ 

[1] Department of Reproduction,Nippon Veterinary and Animal Science University,7-1,Kyonan-cho 1 chome,Musashino-shi,Tokyo 180-8602 Japan

关键词: artificial insemination;    feline;    fresh semen;    vagina;   

DOI  :  10.1292/jvms.62.1163

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(18)Cited-By(29)To clarify the sperm count required for fertilization by artificial intravaginal insemination(AIVI), twenty−nine female cats were examined.Six male cats aged 2−12 years with normal semen quality, copulation capability, and fertility were used.In AIVI, animals received administration of 250 iu hCG once or 100 iu twice on days 2−4 of estrus to induce ovulation, and were inseminated 15, 20, or 30 hr after the initial hCG administration.The success of ovulation was judged by elevation of the peripheral progesterone level after hCG administration.AIVI was investigated at three sperm counts, 20×106(Experiment 1), 40×106(Experiment 2), and 80×106(Experiment 3), with semen collected by the artificial vagina method.Semen was infused in the vagina under general anesthesia by advancing a 9 cm−long nylon probe with 1.5 mm diameter connected to a 1 ml syringe in the vagina for 3−4cm.Ovulation was induced in 43 of 45 animals(95.6%).One of 16 animals was fertilized(conception rate:6.6%)by AIVI in Experiment 1.In Experiments 2 and 3, conception was obtained in six of 18 animals(33.3%)and seven of nine animals(77.8%), respectively, and the mean numbers of kits were 4.0±0.4 and 3.3±0.5, respectively, and the mean numbers of kits were 4.0±0.4(SE)and 3.3±0.5, respectively, showing no significant difference.There were no differences in the time of insemination after hCG administration and the conception rate among these groups.Our findings showed that the number of sperm required for fertilization by AIVI of fresh semen in cats was 80×106.

【 授权许可】

Unknown