Genetic population structure of marine viral haemorrhagic septicaemia virus (VHSV)‡

Diseases of Aquatic Organisms

J. a. King¹  N. Bain¹  R. S. Raynard¹  J. Black¹  H. F. Skall¹  V. Taupin¹  M. Snow¹  C. O. Cunningham¹ 

关键词: Viral Haemorrhagic Septicaemia Virus;    VHSV;    VHS;    Nucleoprotein;    Phylogenetics;   

DOI  :  10.3354/dao061011

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: The nucleotide sequences of a specific region of the nucleoprotein gene were compared in order to investigate the genetic population structure of marine viral haemorrhagic septicaemia virus (VHSV). Analysis of the sequence from 128 isolates ofdiverse geographic and host origin renders this the most comprehensive molecular epidemiological study of marine VHSV conducted to date. Phylogenetic analysis of nucleoprotein gene sequences confirmed the existence of the 4 major genotypes previouslyidentified based on N- and subsequent G-gene based analyses. The range of Genotype I included subgroups of isolates associated with rainbow trout aquaculture (Genotype Ia) and those from the Baltic marine environment (Genotype Ib) to emphasise therelatively close genetic relationship between these isolates. The existence of an additional genotype circulating within the Baltic Sea (Genotype II) was also confirmed. Genotype III included marine isolates from around the British Isles in addition tothose associated with turbot mariculture, highlighting a continued risk to the development of this industry. Genotype IV consisted of isolates from the marine environment in North America. Taken together, these findings suggest a marine origin of VHSV inrainbow trout aquaculture. The implications of these findings with respect to the future control of VHSV are discussed. The capacity for molecular phylogenetic analysis to resolve complex epidemiological problems is also demonstrated and its likely futureimportance to disease management issues highlighted.

【 授权许可】

Unknown