Porcine Granulocyte-Macrophage Colony-Stimulating Factor Improves the in vitro Development of Cloned Porcine Embryos

Journal of Veterinary Medical Science

Seong-Sung KWAK¹  Yubyeol JEON¹  Sang-Hwan HYUN¹  Seung-A CHEONG¹ 

[1] Laboratory of Veterinary Embryology and Biotechnology, College of Veterinary Medicine, Chungbuk National University, Cheongju 361–763, Republic of Korea

关键词: cytokine;    embryo culture;    embryo manipulation;    embryonic development;    swine.;   

DOI  :  10.1292/jvms.12-0050

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(47)Cited-By(7)We examined the effects of porcine granulocyte-macrophage colony-stimulating factor (pGM-CSF) on the in vitro development of porcine embryos produced by somatic cell nuclear transfer (SCNT) for the first time. We evaluated the effects of pGM-CSF on SCNT-derived blastocyst formation and investigated gene expression. A total of 522 cloned embryos in 6 replicates were treated with 10 ng/ml pGM-CSF during in vitro culture (IVC). This treatment significantly (Png/ml pGM-CSF significantly increased POU5F1 and Cdx2 mRNA expression in blastocysts. In addition, Bcl-2, Dnmt1 and proliferating cell nuclear antigen (PCNA) mRNA expression were upregulated in blastocysts in the pGM-CSF supplemented group compared with the control. These results suggest that pGM-CSF improves the quality and developmental viability of porcine SCNT embryos by regulating transcription factor expression.

【 授权许可】

Unknown