Application of crude and recombinant ELISAs and immunochromatographic test for serodiagnosis of animal trypanosomosis in the Umkhanyakude district of KwaZulu-Natal province, South Africa

Journal of Veterinary Medical Science

Mono Sophie MOTSIRI²  Moeti Oriel TAIOE²  Oriel Matlhahane Molifi THEKISOE²  Yasuyuki GOTO³  Shin-Ichiro KAWAZU¹  Moses Sibusiso MTSHALI²  Noboru INOUE¹  Thu-Thuy NGUYEN¹ 

[1] OIE Reference Laboratory for Surra, OIE Collaborating Centre, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan;Parasitology Research Program, Department of Zoology and Entomology, University of the Free State–Qwaqwa Campus, Private Bag, X13, Phuthaditjhaba 9866, South Africa;Laboratory of Molecular Immunology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1�?1�?1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan

关键词: animal African trypanosomosis;    ELISA;    immunochromatographic test;    serodiagnosis;    South Africa;   

DOI  :  10.1292/jvms.14-0330

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(22)Cited-By(2)A total of 231 serum samples were collected from sheep (n=9), goats (n=99) and cattle (n=123) in northeastern KwaZulu-Natal, South Africa. Trypanosome infection was detected using Trypanosoma brucei brucei crude antigen (TbbCA) and T. congolense crude antigen (TcoCA) ELISA assays. Recombinant antigen (T. evansi GM6 which consisted of 4 repeat domains, TeGM6-4r) ELISA and immunochromatographic test (ICT) were also used. Crude antigen ELISA, TeGM6-4r-ELISA and ICT detected 27.3%, 29% and 19.9% of trypanosome seropositive samples, respectively. Trypanosome infection prevalence in cattle and goats was 35.8�?46.3% and 0�?9.1%, respectively. Out of 9 sheep serum samples, 2�?4 sera (22.2�?44.4%) were positive. The detection performance of crude and recombinant antigen ELISAs was relatively similar (K=0.6�?0.7); both are recommended for reference diagnosis and large scale epidemiological surveys. There is potential application for ICT in on-site diagnosis, but its sensitivity should be improved.

【 授权许可】

Unknown