Construction of Swine-Specific CpG Motif Enriched Plasmid and the Study of Its Immunostimulatory Effects Both In Vitro and In Vivo

Journal of Veterinary Medical Science

Hongfei ZHU²  Weifeng YUAN²  José Manuel SÁNCHEZ-VIZCAÍNO⁴  Quan ZHANG¹  Gang ZHU³  Hong JIA²  Xiaoyu GUO²  Shaohua HOU²  Yao SUN⁵ 

[1] College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China;Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China;Xinjiang Tiankang Animal Science Bio-Technology Co. Ltd., Urumqi, 830000, China;Centro de Vigilancia Sanitaria Veterinaria (VISAVET) and Animal Health Department, Complutense University of Madrid, Avda. Puerta de Hierro, S/N 28040 Madrid, Spain;China Animal Husbandry Group Research Institute, Beijing 100081, China

关键词: antibody titer;    CpG motif;    FMD killed vaccine;    lymphocyte transformation test;    stimulation Index;   

DOI  :  10.1292/jvms.11-0543

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(24)A swine-specific CpG motif enriched plasmid (pUC18-CpG) was constructed in this study. Its immunostimulant property was tested in vitro via lymphocyte transformation assay using swine peripheral blood mononuclear cells (PBMCs). The recombinant plasmid showed higher Stimulation Index (SI) compared to the positive control (LPS). In a following animal experiment, pUC18-CpG was co-administered with a commercial swine FMD killed vaccine. Animals in the pUC18-CpG adjuvanted groups showed much higher antibody titers during the vaccination period.

【 授权许可】

Unknown