Epidemiological Survey of Theileria orientalis Infection in Grazing Cattle in the Eastern Part of Hokkaido, Japan

Journal of Veterinary Medical Science

Ikuo IGARASHI²  Naomi OTA²  Kei FUJII³  Shirou MATSUI⁴  Masao KOGA⁴  Naoaki YOKOYAMA²  Hidenari YAMASHINA⁸  Hisashi INOKUMA⁶  Sadao ONOE³  Noritaka KUBOKI²  Teruko HANZAIKE¹  Daisuke MIZUNO²  Hiroshi HATA⁷  Kotaro MATSUMOTO⁶  Seiji KONDO⁷  Yukio NAKAMURA⁵ 

[1] Hokkaido Tokachi Livestock Hygiene Service Center;National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine;Hokkaido Animal Research Center;The National Livestock Breeding Center;Nakamura Domestic Animal Clinic;Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine;Field Science Center for Northern Biosphere, Hokkaido University;Hokkaido Agricultural Developmental Corporation

关键词: cattle;    Hokkaido;    MPSP;    p23;    Theileria orientalis;   

DOI  :  10.1292/jvms.71.937

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(28)Cited-By(48)Theileria orientalis is one of the benign species of Theileria that is widely distributed in Japan and is sometimes responsible for serious economic losses in the livestock industry. In the present study, we surveyed the current status of T. orientalis infection in grazing cattle in the eastern areas of Hokkaido (Taiki, Otofuke, Shintoku, and Shin-Hidaka districts) using molecular methods, as well as traditional methods, of diagnosis. The genes encoding the major piroplasm surface protein (MPSP) and p23 of T. orientalis were identified using highly detectable polymerase chain reaction (PCR). Results of the MPSP-PCR assay indicated that grazing cattle in these districts, after about 1.5 months pasturage, showed high rates of infection, ranging from 10.0-64.8%. Although the main MPSP and p23 genotypes detected were the Ikeda- or Chitose-types, an MPSP gene closely relating to that found in Okinawa prefecture, and a p23 gene closely relating to the Australian (Warwick) Buffeli-type gene, were found in the cattle in Shintoku and Shin-Hidaka districts. The present survey indicated that there were at least five types of T. orientalis classified by their MPSP genes in Hokkaido, Japan, and that T. orientalis infection rates are still high in this region.

【 授权许可】

Unknown