Quantitative PCR detection of feline morbillivirus in cat urine samples

Journal of Veterinary Medical Science

Tetsuya FURUYA⁷  Tsutomu OMATSU⁷  Tomohiro YAMAGUCHI²  Yosuke UEMATSU²  Akiko WACHI⁶  Ryuji FUKUSHIMA³  Yukiko SASSA⁴  Makoto NAGAI⁷  Kinji SHIROTA⁵  Tetsuya MIZUTANI⁷  Makoto SHIBUTANI¹ 

[1] Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture & Technology, 3�?5�?8 Saiwai-cho, Fuchu-shi, Tokyo 183�?8509, Japan;Canine-Lab. Inc., 2�?24�?16 Naka-cho, Venture-Port 301, Koganei-shi, Tokyo 184�?0012, Japan;Laboratory of Veterinary Surgery, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture & Technology, 3�?5�?8 Saiwai-cho, Fuchu-shi, Tokyo 183�?8509, Japan;Laboratory of Epizootiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture & Technology, 3�?5�?8 Saiwai-cho, Fuchu-shi, Tokyo 183�?8509, Japan;Laboratory of Veterinary Pathology, School of Veterinary Medicine, Azabu University, 1�?17�?71 Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa 252�?5201, Japan;Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture & Technology, 3�?5�?8 Saiwai-cho, Fuchu-shi, Tokyo 183�?8509, Japan;Research and Education Center for Prevention of Global Infectious Diseases of Animals, Faculty of Agriculture, Tokyo University of Agriculture & Technology, 3�?5�?8 Saiwai-cho, Fuchu-shi, Tokyo 183�?8509, Japan

关键词: detection;    feline;    paramyxovirus;    PCR;   

DOI  :  10.1292/jvms.15-0112

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(5)Cited-By(1)Feline morbillivirus (FmoPV) is a new virus species and its detection is important, since correlation has been reported between FmoPV virus infection and tubulointerstitial nephritis in cats. Here, we report a real-time reverse transcription (RT)-PCR system that can detect the FmoPV L-gene sequence with more than 10-time higher sensitivity than a conventional PCR system, resulting in detection of less than 10 copies of the template DNA. The total FmoPV positive rate of urine samples from veterinary clinics and hospitals in Japan was 15.1% (25/166) using this system. This study demonstrates usefulness of the real-time RT-PCR system for detection of FmoPV for cat urine samples.

【 授权许可】

Unknown