Prevalence of Mollicutes in Cell Cultures: experience in Cuba

Revista de Salud Animal

Lobo Rivero, Evelyn¹  Burgher Pulgarón, Yaima¹  Pérez Castillo, Anisleidy¹  Timenetsky, Jorge²  Duque Ortiz, Arianna¹  Miranda Marques, Lucas² 

[1] Centro Nacional de Sanidad Agropecuaria (CENSA), San José de las Lajas, Cuba;Universidade de São Paulo, Brazil

关键词: mollicutes;    Mycoplasmas;    Acholeplasma;    cell cultures;    diagnostic;   

DOI  :  

学科分类：兽医学

来源: Centro Nacional de Sanidad Agropecuaria

【 摘 要 】

Different mollicutes species of the Mycoplasma and Acholeplasma genera can contaminate the cell cultures and raw materials commonly used in the manufacture of a variety of biological and therapeutical products. In this study, the presence of mollicutes was detected in 19 of 50 cell culture samples analyzed using PCR and microbiological cultures. The species most frequently detected was M. fermentans (31.5%), followed by A. laidlawii (26.3%), M. orale (15.7%), M. hyorhinis (15.7%), and M. salivarium (10, 5%). A. laidlawii was present as the infecting agent only in 10.5% of the samples. These results showed the main species of contaminants, which suggested mollicutes multiple origin in the source of infection.

【 授权许可】

CC BY-NC