Establishment of serological test to detect antibody against ferret coronavirus

Journal of Veterinary Medical Science

Akihiko OTA¹  Kaneichi NAKAMURA^1,12  Ken MAEDA⁹  Masaki TAKIZAWA²  Kenichi TAMUKAI^1,13  Hiroshi SHIMODA⁹  Masanari OHASHI^1,14  Yoshihito AKABANE^1,10  Jouji OHTA³  Masao OHWAKI³  Yutaka TERADA⁹  Shohei MINAMI⁹  Eiichi KANAGAWA^1,11  Yoshinori TAKAMI⁸  Yuichi OTA¹  Yumiko NAGANUMA⁵  Mamoru ONUMA⁷  Yumi UNE⁶  Yasutsugu MIWA⁴  Keiichiro WATANABE⁵  Tomoaki TANOUE³ 

[1] Tenpaku Animal Hospital, 1�?2011 Hirabariminami, Tenpaku, Nagoya City, Aichi 468�?0020, Japan;Takizawa Animal Hospital, 2�?95�?3 Miyahara-cho, Kita, Saitama 331�?0812, Japan;Inuyama Animal General Medical Center, 29 Haguroohmishita, Inuyama, Aichi 484�?0894, Japan;Miwa Exotic Animal Hospital, 1�?25�?5 Komagome, Toshima, Tokyo 170�?0003, Japan;Japan Animal Medical Center, 6�?22�?3 Honmachi, Shibuya, Tokyo 151�?0071, Japan;Laboratory of Veterinary Pathology, School of Veterinary Medicine, Azabu University, 1�?17�?71 Fuchinobe, Chuo, Sagamihara, Kanagawa 252�?5201, Japan;Oosagami Animal Clinic, 1�?33�?3 Laketown, Koshigaya, Saitama 343�?0828, Japan;Verts Animal Hospital, 2�?21�?5 Naka, Hakata, Fukuoka 812�?0893, Japan;Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677�?1 Yoshida, Yamaguchi 753�?8515, Japan;Jinryo Being Animal Hospital, 2�?8�?3 Jinryo-cho, Kasugai, Aichi 486�?0821, Japan;Maruko Mirai Animal Hospital, 3�?22�?4 Shimomaruko, Ota, Tokyo 146�?0092, Japan;Nakamura Pet Clinic, 4�?5�?30 Sakurabashi, Naka, Okayama 703�?8285, Japan;Denenchofu Animal Hospital, 2�?1�?3 Denenchofu, Ota, Tokyo 145�?0071, Japan;Ohashi Animal Hospital, 1950�?2 Goudonishi, Iwasaki, Komaki, Aichi 485�?0011, Japan

关键词: enzyme-linked immunosorbent assay (ELISA);    ferret coronavirus (FRCoV);    nucleocapsid (N);   

DOI  :  10.1292/jvms.16-0059

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(13)Cited-By(1)Since there is no available serological methods to detect antibodies to ferret coronavirus (FRCoV), an enzyme-linked immunosorbent assay (ELISA) using recombinant partial nucleocapsid (N) proteins of the ferret coronavirus (FRCoV) Yamaguchi-1 strain was developed to establish a serological method for detection of FRCoV infection. Many serum samples collected from ferrets recognized both a.a. 1�?179 and a.a. 180�?374 of the N protein, but two serum samples did not a.a. 180�?374 of the N protein. This different reactivity was also confirmed by immunoblot analysis using the serum from the ferret.Therefore, the a.a. 1�?179 of the N protein was used as an ELISA antigen. Serological test was carried out using sera or plasma of ferrets in Japan. Surprisingly, 89% ferrets in Japan had been infected with FRCoV. These results indicated that our established ELISA using a.a. 1�?179 of the N protein is useful for detection of antibody to FRCoV for diagnosis and seroepidemiology of FRCoV infection.

【 授权许可】

Unknown