Epidemiological Analysis of Nosocomial MRSA Outbreaks Using Phage Open-Reading Frame Typing in a Tertiary-Care Hospital

Japanese Journal of Infectious Diseases

Koichi Tokuda⁴  Hideki Kawamura⁴  Toyoyasu Koriyama⁴  Tomohiro Kubota³  Teruto Hashiguchi²  Junichiro Nishi⁴  Yoshifumi Kawano³  Hiroaki Miyanohara²  Naoko Imuta¹ 

[1] Department of Microbiology, Kagoshima University Graduate School of Medical and Dental Sciences;Clinical Laboratory, Kagoshima University Hospital;Department of Pediatrics, Kagoshima University Graduate School of Medical and Dental Sciences;Department of Infection Control and Prevention, Division of Medical and Environmental Safety, Kagoshima University Hospital

关键词: genotyping;    methicillin-resistant Staphylococcus aureus;    outbreak;    pulsed-field gel electrophoresis;    phage open-reading frame typing;   

DOI  :  10.7883/yoken.JJID.2015.320

学科分类：传染病学

来源: National Institute of Infectious Diseases

【 摘 要 】

References(4)Pulsed-field gel electrophoresis (PFGE) is a reliable method for analyzing outbreaks of methicillin-resistant Staphylococcus aureus (MRSA); however, it is time-consuming and technically demanding. A new strain-differentiation method for MRSA, namely phage open reading frame (ORF) typing (POT), is a rapid PCR-based technique, in which the ORFs of lysogenized phage genomes in MRSA are amplified. The aim of this study was to evaluate the utility of the POT method for epidemiological analysis of nosocomial MRSA outbreaks. Forty-four strains from 12 episodes of 3 or more nosocomial MRSA infections in 1 ward within a 4-week period were characterized using PFGE and POT methods. The strains were classified into 16 distinct types using POT and 19 subtypes using PFGE. We defined an outbreak as 3 or more new MRSA infections caused by strains with indistinguishable genetic patterns. The identification of 11 (91.7%) episodes by PFGE, including 4 outbreaks and 7 sporadic events, was consistent with the results of POT analysis. These results suggest that POT is a useful epidemiological tool for evaluating nosocomial MRSA outbreaks.

【 授权许可】

Unknown