Tumor Promoting Effect of Phenolphthalein on Development of Lung Tumors Induced by N-ethyl-N-nitrosourea in Transgenic Mice Carrying Human Prototype c-Ha-ras Gene

Journal of Veterinary Medical Science

Hiroshi ONODERA¹  Yoko KASHIDA²  Takao WATANABE²  Masao HIROSE¹  Masako IMAOKA²  Kunitoshi MITSUMORI²  Makoto UEDA¹ 

[1] Division of Pathology, National Institute of Health Sciences;Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology

关键词: lung tumor;    phenolphthalein;    rasH2 mouse;    tumor promotion;   

DOI  :  10.1292/jvms.64.489

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(17)Cited-By(2)In order to examine tumor modifying effects of phenolphthalein (PhP), female transgenic mice carrying human prototype c-Ha-ras gene (rasH2 mice) were given a single intraperitoneal injection of 60 mg/kg body weight of N-ethyl-N-nitrosourea (ENU), followed by the diet containing 12,000 ppm PhP for 26-week. Histopathologically, alveolar hyperplasias, adenomas and adenocarcinomas were observed in the ENU + PhP group, but only hyperplasias and adenomas were observed in the ENU alone group. The incidence and multiplicity of adenocarcinomas in the ENU + PhP group was significantly increased as compared to that in the ENU alone group. The combined multiplicity of adenomas and adenocarcinomas in this group was also significantly higher than that of the ENU alone group. In addition, the ratio of area of adenomas in the ENU + PhP group was significantly higher than that in the ENU alone group. The result of our study suggests that PhP has a clear tumor promoting effect in the lung of rasH2 mice.

【 授权许可】

Unknown