Suppression of Transcription Activity of the MEQ Protein of Oncogenic Marek's Disease Virus Serotype 1 (MDV1) by L-MEQ of Non-Oncogenic MDV1

Journal of Veterinary Medical Science

Kazuhiko OHASHI¹  Kyung-Soo CHANG¹  Misao ONUMA¹ 

[1] Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University

关键词: dual luciferase assay;    L-meq;    Marek's disease virus;    meq;    transcription activity;   

DOI  :  10.1292/jvms.64.1091

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(25)Cited-By(14)meq is one of the candidate oncogenes in the MDV1 genome. We previously reported a difference in the meq open reading frame (ORF) between oncogenic and non-oncogenic MDV1: L-meq, in which a 180-bp sequence is inserted into the meq ORF, is detected in non-oncogenic MDV1. To study the functions of a gene product of L-meq (L-MEQ), transactivation by L-MEQ was analyzed by dual luciferase assay using a reporter gene under the control of long (-1--873 bp) and short (-1--355 bp) meq promoter (LMP and SMP, respectively). LMP showed higher promoter function than SMP. L-MEQ transactivated the expression of the reporter gene, but less than MEQ did. In the presence of SMP or the cytomegalovirus immediate-early promoter, the same or slightly higher transactivation was observed in cells cotransfected with both meq and L-meq than cells transfected only with meq. However, in the presence of LMP, lower transactivation was observed in cells cotransfected with both meq and L-meq than cells transfected only with meq, suggesting that L-MEQ can be a transrepressor. Replication of a vvMDV1 was enhanced in the cells with meq. Interestingly, however, replication of vvMDV1 was suppressed in the cells with L-meq or with both L-meq and meq, compared to untransfected cells. Thus, L-MEQ could suppress replication of vvMDV1 displaying the meq gene in coinfetced cells.

【 授权许可】

Unknown