Bee Venom Induces Apoptosis and Inhibits Expression of Cyclooxygenase-2 mRNA in Human Lung Cancer Cell Line NCI-H1299

Journal of Pharmacological Sciences

Mi-Hyeon Jang³  Hi-Joon Park²  Sabina Lim²  Min-Chul Shin³  Insop Shin²  Ji-Suk Lee²  Kyoung-Ah Kim²  Chang-Ju Kim³  Seung-Moo Han²  Ee-Hwa Kim¹ 

[1] Department of Meridianology, College of Oriental Medicine, Semyung University;Research Group of Pain and Neuroscience in Vision 2000 Project, East-West Medical Research Institute, Kyung Hee Universiy;Department of Physiology, College of Medicine, Kyung Hee University

关键词: bee venom;    lung cancer;    apoptosis;    cyclooxygenase;    prostaglandin E2;   

DOI  :  10.1254/jphs.91.95

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(37)Cited-By(55)To investigate whether bee venom (BV) induces apoptosis, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, terminal deoxynucleotidyl transferase- mediated dUTP nick end-labeling assay, 4,6-diamidino-2-phenylindole staining, flow cytometric analysis, and DNA fragmentation assay were performed on NCI-H1299 lung cancer cells treated with BV. Through morphological and biochemical analyses, it was demonstrated that NCI-H1299 cells treated with BV exhibit several features of apoptosis. In addition, reverse transcription-polymerase chain reaction and prostaglandin E2 (PGE2) immunoassay were performed to verify whether BV possesses an inhibitory effect on the expression of cyclooxygenase (COX) and PGE2 synthesis. Expression of COX-2 mRNA and synthesis of PGE2 were inhibited by BV. These results suggest the possibility that BV may exert an anti-tumor effect on human lung cancer.

【 授权许可】

Unknown