Detection and surveillance of viral hemorrhagic septicemia virus using real-time RT-PCR. II. Diagnostic evaluation of two protocols

Diseases of Aquatic Organisms

Emily R. Cornwell¹  Robert Kim¹  Kathy Toohey-Kurth¹  Mohamed Faisal¹  Andrew E. Goodwin¹  Geoffrey H. Groocock¹  Monica M. Reising¹  Cem Giray¹  Gwenn E. Merry¹  0¹  Isaac Standish¹  Nicholas B. D. Phelps¹  Janet V. Warg¹  Rodman G. Getchell¹  Yan Zhang¹  Travis Clement¹  Angela Cruz¹ 

关键词: VHSV;    Surveillance;    Real-time RT-PCR;    Diagnostic sensitivity;    Diagnostic specificity;   

DOI  :  10.3354/dao02758

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Two real-time reverse transcription polymerase chain reaction (rRT-PCR) assays under consideration for deployment to multiple testing laboratories across the USA were evaluated for diagnostic sensitivity and specificity on tissue homogenates obtained from natural and experimental viral hemorrhagic septicemia (VHS)-infected fish. Estimates for diagnostic specificity using virus isolation as the reference method were similar between laboratories regardless of the assay. Diagnostic sensitivity estimates of 0.96 (95% CI: 0.95, 0.97) for Jonstrup et al. (2013)’s assay (J Fish Dis 36:9-23) exceeded the diagnostic sensitivity of 0.85 (95% CI: 0.83, 0.87) for Phelps et al. (2012)’s assay (J Aquat Anim Health 24:238-243). The Jonstrup rRT-PCR assay is robust as demonstrated by high sensitivity and specificity estimates across laboratories and can be used as a valuable tool for targeted surveillance and for testing of suspect VHSV samples.

【 授权许可】

Unknown