期刊论文详细信息
Diseases of Aquatic Organisms
Detection and surveillance of viral hemorrhagic septicemia virus using real-time RT-PCR. II. Diagnostic evaluation of two protocols
Emily R. Cornwell1  Robert Kim1  Kathy Toohey-Kurth1  Mohamed Faisal1  Andrew E. Goodwin1  Geoffrey H. Groocock1  Monica M. Reising1  Cem Giray1  Gwenn E. Merry1  01  Isaac Standish1  Nicholas B. D. Phelps1  Janet V. Warg1  Rodman G. Getchell1  Yan Zhang1  Travis Clement1  Angela Cruz1 
关键词: VHSV;    Surveillance;    Real-time RT-PCR;    Diagnostic sensitivity;    Diagnostic specificity;   
DOI  :  10.3354/dao02758
学科分类:生物科学(综合)
来源: Inter-Research
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【 摘 要 】

ABSTRACT: Two real-time reverse transcription polymerase chain reaction (rRT-PCR) assays under consideration for deployment to multiple testing laboratories across the USA were evaluated for diagnostic sensitivity and specificity on tissue homogenates obtained from natural and experimental viral hemorrhagic septicemia (VHS)-infected fish. Estimates for diagnostic specificity using virus isolation as the reference method were similar between laboratories regardless of the assay. Diagnostic sensitivity estimates of 0.96 (95% CI: 0.95, 0.97) for Jonstrup et al. (2013)’s assay (J Fish Dis 36:9-23) exceeded the diagnostic sensitivity of 0.85 (95% CI: 0.83, 0.87) for Phelps et al. (2012)’s assay (J Aquat Anim Health 24:238-243). The Jonstrup rRT-PCR assay is robust as demonstrated by high sensitivity and specificity estimates across laboratories and can be used as a valuable tool for targeted surveillance and for testing of suspect VHSV samples.

【 授权许可】

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