In Vivo and In Vitro Transcription of Small mRNAs Containing a Leader Sequence from Mouse Hepatitis Virus Strain JHM

Journal of Veterinary Medical Science

Akihiko MAEDA¹  Tomomasa WATANABE¹  Masanobu HAYASHI¹  Kozue ISHIDA¹  Shigeo NAMIOKA¹  Tetsuya MIZUTANI¹ 

关键词: C57BL/6 mouse;    MHV;    small RNA;   

DOI  :  10.1292/jvms.56.1013

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(15)Two additional small RNAs, named mRNA8 and 9, are transcribed from mouse hepatitis virus (MHV) in virus-infected mouse DBT cells. This report shows that the small mRNAs (mRNA8 and 9) were observed at 3 hr post infection (p. i. ) in DBT cells infected with the JHM strain of MHV. This result suggested that products from mRNA8 and 9 may play a role in the early stage of the viral replication cycle in the infected DBT cells. The mRNA8 is initiated from a perfectly conserved intergenic site, but mRNA9 is from an imperfectly conserved intergenic sequence. Since mRNA8 and 9 were found in the liver flnd brain of an infected mouse, it was suggested that the imperfect intergenic sequence of MHV may serve as an initiation site for leader-primed transcription in vivo.

【 授权许可】

Unknown