Reactivity of Anti-Nipah Virus Monoclonal Antibodies to Formalin-fixed, Paraffin-embedded Lung Tissues from Experimental Nipah and Hendra Virus Infections

Journal of Veterinary Medical Science

John WHITE¹  Aziz JAMALUDDIN²  Shahirudin SHAMUSUDIN²  Yoshihito KASHIWAZAKI²  Gail RUSSELL¹  Tadao IMADA³  Nobuhiko TANIMURA³  Sharifah SYED HASSAN² 

[1] CSIRO, Livestock Industries, Australian Animal Health Laboratory;Veterinary Research Institute, Department of Veterinary Services;National Institute of Animal Health

关键词: immunohistochemistry;    monoclonal antibody;    Nipah virus;   

DOI  :  10.1292/jvms.66.1263

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(16)Cited-By(6)The immunohistochemical reactivity of seven clones of mouse monoclonal antibodies raised to Nipah virus antigens were investigated using formalin-fixed, paraffin embedded porcine and equine lung tissues from experimental Nipah and Hendra virus infection, respectively. Either microwave irradiation or enzymatic digestion effectively unmasked the viral antigens in formalin-fixed, paraffin-embedded tissue sections. Four clones showed positive reaction to both Nipah virus-infected porcine lung tissue and Hendra virus-infected equine lung tissue. Two clones (11F6 and 13A5) reacted with Nipah virus-infected porcine lung tissue, but not with Hendra virus-infected equine lung tissue. These Nipah virus-specific monoclonal antibodies may therefore be useful for immunohistological diagnosis of Nipah virus infection and for further research on Nipah virus pathogenesis.

【 授权许可】

Unknown