Establishment of a Cell Line Persistently Infected with Chronic Wasting Disease Prions

Journal of Veterinary Medical Science

Hyun-Joo SOHN¹  Dong-Seob TARK¹  Yoon-Hee LEE¹  Min-Jeong KIM¹  Won-Yong LEE¹  Takashi YOKOYAMA²  In-Soo CHO¹  Hyo-Jin KIM¹ 

[1] Foreign Animal Disease Division, Animal Plant and Fisheries Quarantine and Inspection Agency, Anyang, Gyeonggi-do 430–757, Korea;Prion Disease Research Center, National Institute of Animal Health, Tsukuba, Ibaraki 305–0856, Japan

关键词: CWD;    infection;    lentivirus;    prion;    RK13 cells;   

DOI  :  10.1292/jvms.12-0061

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(16)Cited-By(4)Elk prion protein (PrPC) has been confirmed to be capable of rendering rabbit epithelial RK13 cells permissive to temporal infection by chronic wasting disease (CWD) prions. The present study satisfactorily generated persistently CWD prion-affected RK13 cells (RKC1-11) using elk PrPC expressing cells (elkRK13) that were generated via the lentiviral expression system with high efficiency. The elkRK13 cells have been shown to be permissive to accumulation of abnormal isoforms of prion protein (PrPSc) resulting from CWD prions up to 97 serial passages thus far. This novel prion-affected cell line will help facilitate investigation of the molecular basis of CWD prion pathogenesis and confirmation of CWD prion infectivity in vitro.

【 授权许可】

Unknown