Establishment and Characterization of Two Cell Lines Derived from Canine Spontaneous Osteosarcoma

Journal of Veterinary Medical Science

Sung-Hyeok HONG³  Satoru MATSUNAGA²  Tsuyoshi KADOSAWA¹  Nobuo SASAKI³  Ryohei NISHIMURA³  Manabu MOCHIZUKI³ 

[1] Laboratory of Veterinary Surgery, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University;Veterinary Medical Center, Faculty of Agriculture, The University of Tokyo;Laboratory of Veterinary Surgery, Division of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo

关键词: canine;    cell line;    osteosarcoma;   

DOI  :  10.1292/jvms.60.757

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(19)Cited-By(15)Two new canine osteosarcoma cell lines were established. One (OOS) was established from a 10-year-old female maltese dog with mandibular osteosarcoma and the other (HOS) from a 7-year-old male mongrel dog with scapular osteosarcoma. Histopathological types of OOS and HOS were mixed and fibroblastic cell type, respectively. Transmission electron microscopic features of HOS revealed prominent rough endoplasmic reticulum, suggesting higher malignancy comparing to OOS. Doubling time of OOS and HOS were 45.0 ± 0.5 hr and 42.0 ± 0.1 hr, respectively. Alkaline phosphatase activities of OOS and HOS were quite low. Histological features of tumor tissues produced by transplantation of these cells into nude mice were identical to those of original osteosarcomas.

【 授权许可】

Unknown