PCR-Based Restriction Fragment Length Polymorphism (RFLP) Analysis of Campylobacter jejuni Isolates from Humans, Chickens and Dogs in Northern Taiwan

Journal of Veterinary Medical Science

Hsiang-Jung TSAI²  Huei-Lin TSAI¹  Huang-Chi HUANG²  Cha-Chin CHANG¹ 

[1] Mackay Memorial Hospital;Department of Veterinary Medicine, National Taiwan University

关键词: Campylobacter jejuni;    canine;    chicken;    human;    PCR-RFLP analysis;   

DOI  :  10.1292/jvms.68.815

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(35)Cited-By(1)Two hundred and twenty strains of Campylobacter jejuni (70 human, 51 canine and 99 chicken strains) were isolated from September 2003 to September 2004 in northern Taiwan. These strains were subtyped by PCR-RFLP analysis of the flagellin (FlaA) gene. On the basis of restrictive digest, six types were identified with AfaI, seven types with MboI and five types with HaeIII. With the combination of these three enzymes, 47 distinct PCR-RFLP patterns were observed-25 each from human and chicken isolates, and 9 from canine isolates. In human strains, the most frequently occurring types were Cj-28 (14.3%), Cj-17 (10%), Cj-16 (8.6%), Cj-37 (7.1%) and Cj-46 (7.1%). In canine strains, the most prevalent types were Cj-1 (33.3%), Cj-26 (19.6%), Cj-3 (15.7%), Cj-2 (9.8%) and Cj-10 (9.8%). In chicken strains, the most frequently occurring types were Cj-46 (40.4%), Cj-29 (9.1%), Cj-45 (7.1%) and Cj-41 (5.1%). The results suggest that poultry is a source, but not the sole source, of C. jejuni infection in humans. Two RFLP types, Cj-17 and Cj-37, frequently occurring in human isolates in this study have also been found to be prevalent in human isolates in Japan, China and the Czech Republic, indicating a possible international clonal spread.

【 授权许可】

Unknown