Effects of blood anticoagulants on stable isotope values of sea turtle blood tissue

Aquatic Biology

†¹  Garrett E. Lemons, Tomoharu Eguchi, Boyd N. Lyon¹  Robin LeRoux, Jeffrey A. Seminoff¹ 

关键词: δ13C and δ15N;    Carbon;    Cheloniidae;    Ectotherm;    ­Isotope enrichment;    Nitrogen;    Reptilia;   

DOI  :  10.3354/ab00397

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Collecting tissue samples from sea turtles for stable isotope analysis often occurs at remote field sites. For blood tissue, samples are treated with an anticoagulant that allows for later separation of plasma from cellular components. However, the effect of this technique on stable isotope values of sea turtle blood has not been established. We measured the effects of 3 widely used anticoagulants, acid ­citrate dextrose (ACD), sodium heparin (SH) and ­ethylenediaminetetraacetic acid (EDTA), on stable carbon (δ¹³C) and stable nitrogen (δ¹⁵N) values in whole blood, red blood cells, and blood plasma of 11 green turtles Chelonia mydas captured in San Diego Bay, California, USA. Vials containing each of the 3 blood preservatives as well as a vial containing no additive (i.e. control vial) were filled in random order. Blood in the no-additive vial was immediately separated into fractions (e.g. red blood cells, plasma) via centrifugation, whereas blood collected in the treatment vials was chilled and then centrifuged 48 h after collection. We found that, relative to the controls, ACD-preserved whole blood and blood plasma were ¹³C enriched, EDTA-treated red blood cells and plasma were ¹⁵N de­pleted, and SH-treated whole blood was ¹⁵N en­riched. Because SH was the only anticoagulant with no measured effect on blood plasma and red blood cells—the most commonly studied blood fractions for sea turtle stable isotope studies—we recommend its exclusive use as a blood anticoagulant for field studies where prompt centri­fugation is not possible.

【 授权许可】

Unknown