Natural infection of the redclaw crayfish Cherax quadricarinatus with presumptive spawner-isolated mortality virus

Diseases of Aquatic Organisms

Catriona McElnea¹  Leigh Owens¹ 

关键词: Freshwater crayfish;    Cherax quadricarinatus;    Spawner-isolated mortality virus;    Mortalities;   

DOI  :  10.3354/dao040219

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Crayfish farmers reported reduced tolerance of stress in specimens of Cherax quadricarinatus, which were formerly robust crayfish. Furthermore, one farmer reported a large reduction in yield with final harvest only equaling the stockingweight. Upon trapping, one-third of the crayfish regularly died overnight and a further one-third died on the sorting tray during sexing of juveniles (~3 mo old). Histopathological examination revealed very light (1 or 2 cells per section) infections withCherax giardiavirus and sometimes mild atrophy of hepatopancreatic cells. Gene probe analysis with a DIG-labeled spawner-isolated mortality virus (SMV) probe demonstrated extensive positive signals in nuclei of many tissues. The hepatopancreas, themidgut, glands associated with the midgut, the epithelium of seminal ducts and follicle cells surrounding oocytes gave the strongest positive signals. Nuclei of the heart, haemocytes, connective tissue and subcutis gave positive signals in someindividuals. Although signals were intense and extensive, cytolysis of infected cells was very limited. The possibility of cross infections of SMV between prawns and freshwater crayfish is of international quarantine significance.

【 授权许可】

Unknown