Evaluation of Highly Sensitive Thyrotropin Assay for Detecting Thyroid Diseases in Neonatal Screening: Preliminary Studies

Endocrine Journal

YOSHIHIDE NARIZUKA¹  HIROKI TATSUMI²  TOMIKO MIYAGI³  TOSHIAKI OURA⁷  KAZUTAKA INAOKA⁵  KATSUMI TANIGUCHI¹  KIYOSHI MIYAI⁴  SHINOBU IDA⁵  NOBUYUKI ASHIDA⁶ 

[1] Immunochemistry System Department, Eiken Chemical Co., Ltd;Research and Development Division, Kikkoman Corporation;Osaka City Environment and Public Health Association;Koshien University, College of Nutrition;Osaka Medical Center and Research Institute for Maternal and Child Health;School of Allied Health Sciences, Faculty of Medicine, Osaka University;Osaka City Rehabilitational Training Center

关键词: Neonatal screening;    Central hypothyroidism;    Sensitive TSH assay;    Firefly luciferase;   

DOI  :  10.1507/endocrj.45.761

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(16)Cited-By(3)TSH concentrations in dried blood samples on filter paper were determined by a conventional enzyme-linked immunosorbent assay (ELISA), used for routine neonatal screening for primary hypothyroidism, and a highly sensitive bioluminescence ELISA (BL-ELISA) using firefly luciferase to examine whether central hypothyroidism and hyperthyroidism can be efficiently detected as cases of primary hypothyroidism. Samples were obtained from 3 patients with congenital central hypothyroidism, 5 patients with congenital primary hypothyroidism, 6 patients with hyperthyroidism, 31 neonatal babies with low birth weight (premature babies) and 242 newborn babies with normal birth weight from the general population (normal babies). The TSH values were low in central hypothyroidism and hyperthyroidism. Their deviations from the mean TSH value for normal babies by the BL-ELISA method (-3.12 SD and -4.79 SD in central hypothyroidism and hyperthyroidism respectively) were greater than those by the ELISA method (-2.00 SD and -2.97 SD respectively). The TSH values were high in primary hypothyroidism and normal in premature babies while deviations were the same when BL-ELISA and ELISA were used. These findings indicate that the highly sensitive TSH assay (BL-ELISA) can be used for detecting both primary and central hypothyroidism as well as hyperthyroidism in neonatal screening.

【 授权许可】

Unknown