Inhibitory Effects of Glucocorticoids on Proliferation of Canine Mast Cell Tumor

Journal of Veterinary Medical Science

Tomoko TAKAHASHI³  Satoru MATSUNAGA¹  Tsuyoshi KADOSAWA²  Masayuki NAGASE³  Nobuo SASAKI³  Ryohei NISHIMURA³  Manabu MOCHIZUKI³ 

[1] Veterinary Medical Center, Faculty of Agriculture, The University of Tokyo;Laboratory of Veterinary Surgery, Department of Veterinary Clinical Sciences, Graduated School of Veterinary Medicine, Hokkaido University;Laboratory of Veterinary Surgery, Division of Veterinary Medical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo

关键词: canine;    cell line;    glucocorticoid;    glucocorticoid receptor;    mastocytoma;   

DOI  :  10.1292/jvms.59.995

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(27)Cited-By(16)The inhibitory effect of glucocorticoids (GCs) on proliferation of canine mast cell tumor (MCT) was studied using two types of MCT cells; JuMC cells and LuMC cells derived from spontaneous canine cutaneous and intestinal MCT, respectively. In in vitro study, growth of JuMC cells was significantly inhibited with more than 1 nM GCs and apoptotic-like cell death was seen, while that of LuMC cells was never inhibited even with 10 μM GCs. Growth rate of masses in nude mice developed by inoculation of JuMC cells was reduced in a dose-dependent manner by administration of GC, while growth inhibition of masses developed by inoculation of LuMC cells was minimal with increasing GC doses. Competitive binding studies and Scatchard analysis demonstrated the presence of high-affinity, low capacity GC receptors in both JuMC and LuMC cells. Kd was estimated to be 1.30 nM in JuMC cells and 0.45 nM in LuMC cells, respectively. It is concluded that canine cutaneous MCT cells responded to GCs in vitro and in vivo, whereas intestinal MCT cells did not, though both types of cells had specific GC receptors.

【 授权许可】

Unknown