Detection of Panulirus argus Virus 1 (PaV1) in exported frozen tails of subadult-adult Caribbean spiny lobsters Panulirus argus

Diseases of Aquatic Organisms

Cristina Pascual-Jiménez¹  Juan Pablo Huchin-Mian¹  Patricia Briones-Fourzán¹  Raúl Simá-Álvarez¹  Yanis Cruz-Quintana¹  Enrique Lozano-Álvarez¹  Rossanna Rodríguez-Canul¹  Juan Antonio Pérez-Vega¹ 

关键词: Panulirus argus Virus 1;    Frozen lobster tails;    Caribbean Sea;   

DOI  :  10.3354/dao02117

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: The Caribbean spiny lobster Panulirus argus is a valuable fishing resource and the trade in frozen lobster tails is an important industry. However, the presence of the pathogenic virus Panulirus argus Virus 1 (PaV1), which causes systemic infection in P. argus and is particularly lethal to juvenile individuals, has not been previously examined in imported/exported lobster products. We used PCR assays to determine the presence of PaV1 in abdominal muscle tissue of 22 frozen P. argus tails exported from Belize to Mexico. Based on their size, the tails belonged to subadult–adult lobsters. Using specific primers targeted for PaV1 resulted in 11 tails showing a specific 499 bp band. The sequence of positive amplified fragments showed a high similarity to PaV1 (95% identity with GenBank accession no. EF206313.1). Although the pathogenicity of PaV1 was not evaluated in the present study, our results provide the first evidence of PaV1 in frozen lobster tails exported in the seafood industry as well as the first molecular evidence of PaV1 in adult lobsters.

【 授权许可】

Unknown