Atopic NC/Nga Mice as a Model for Allergic Asthma: Severe Allergic Responses by Single Intranasal Challenge with Protein Antigen

Journal of Veterinary Medical Science

Akane TANAKA¹  Atsuko ITAKURA¹  Ken OHTA³  Misao ONUMA²  Naomi YAMASHITA³  Hiroshi MATSUDA¹  Tadashi IWASAKI² 

[1] Laboratory of Clinical Immunology, Department of Veterinary Clinic, Faculty of Agriculture, Tokyo University of Agriculture and Technology;Laboratory of Infectious Diseases, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University;Department of Medicine, Teikyo University School of Medicine

关键词: allergy;    cytokine;    eosinophil;    in vivo animal model;    lung.;   

DOI  :  10.1292/jvms.63.413

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(28)Cited-By(7)Since certain characters of allergic asthma are common with other allergic disorders like atopic dermatitis, the possible relationship in etiology is expected. Herein, we investigated whether NC/Nga mice, an inherent animal model for human atopic dermatitis, are inclined to allergic asthma. A single intranasal challenge of NC/Nga mice immunized with ovalbumin (OVA) resulted in an increase in plasma levels of OVA-specific IgE, and typical pathological aspects of allergic asthma characterized by infiltration of numerous eosinophils, mucus hyper production of bronchial epithelial cells. Moreover, airway hyperresponsiveness to inhaled acetylcholine and marked enhancement of airway resistance after the challenge were observed as compared to control BALB/c mice. Delayed expression of mRNA of eosinophil active chemokines, interleukin-5, eotaxin, macrophage inflammatory protein-1 α in concert with eosinophilia was determined in the lung of NC/Nga mice. These results suggest that asthmatic responses developed in NC/Nga mice challenged with OVA are very similar to human allergic asthma, and that NC/Nga mice are a useful model to elucidate various aspects of allergic asthma.

【 授权许可】

Unknown