Epitope Mapping of a Monoclonal Antibody Specific to Feline Panleukopenia Virus and Mink Enteritis Virus

Journal of Veterinary Medical Science

Masami MOCHIZUKI²  Naotaka ISHIGURO¹  Morikazu SHINAGAWA¹  Hideyuki NAGASAWA³  Motohiro HORIUCHI¹ 

[1] Department of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine;Laboratory of Clinical Microbiology, Kyoritsu Shoji Co.;Research Center for Protozoan Molecular Immunology, Obihiro University of Agriculture and Veterinary Medicine

关键词: canine parvovirus;    feline panleukopenia virus;    monoclonal antibody;   

DOI  :  10.1292/jvms.59.133

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(18)Cited-By(12)To obtain monoclonal antibodies (MAbs) specific to feline panleukopenia virus (FPLV) and mink enteritis virus (MEV), 15 hybridomas secreting MAbs against MEV-Abashiri were established and the properties of the MAbs were analyzed. The cross-reactivity of MAbs revealed that one MAb, P2-215 was specific for FPLV and MEV, whereas the remaining fourteen MAbs reacted with canine parvovirus (CPV), FPLV, and MEV. Epitope analyses using various CPV/MEV chimeric viruses revealed that the MAb P2-215 recognized the epitope comprised of amino acid 93-Lys in VP2, which is known to be FPLV and MEV-specific.

【 授权许可】

Unknown