Journal of Veterinary Medical Science | |
Production of Exfoliative Toxin A by Staphylococcus aureus Isolated from Mastitic Cow’s Milk and Farm Bulk Milk | |
Michiko HAYASHI2  Takeshi SHIMANO2  Nozomu HASHIMOTO3  Hideo IGARASHI1  Kyuhei TAKEUCHI2  Shotaro TAKEUCHI3  Hirofumi KOMAE2  Miyoko ENDOU1  Yuji HAYAKAWA2  | |
[1] Department of Microbiology, Tokyo Meteropolitan Research Laboratory of Public Health;Hokubu Livestock Hygiene Service Center;Department of Bioscience, Faculty of Biotechnology, Fukui Prefectural University | |
关键词: bovine mastitis; exfoliative toxin A; Staphylococcus aureus; | |
DOI : 10.1292/jvms.60.1281 | |
学科分类:兽医学 | |
来源: Japanese Society of Veterinary Science | |
【 摘 要 】
References(19)Cited-By(8)The production of exfoliative toxins A and B (ETA and ETB) by Staphylococcus aureus isolated from mastitic cow's milk and farm bulk milk was examined by the reverse passive latex agglutination method (RPLA). ETA was detected in 2 (1.2%) of 162 isolates from mastitic cow's milk and in 1 (0.6%) of 166 isolates from farm bulk milk. RPLA titers of these isolates were much lower than in human isolates. No ETB was detected in any of the isolates tested. These ETA-positive isolates belonged to bovine ecovar. They were non-typable using the international phage set for human strains. When these ETA-positive isolates were subcutaneously inoculated into neonatal mice, general exfoliation of the epidermis accompanied by the so-called Nikolsky sign was not recognized. By the immunoblotting and PCR methods, however, ETA and eta gene were recognized in the ETA-positive isolates from mastitic cow's milk and farm bulk milk. These data suggest that ETA is also produced by bovine isolates of S. aureus, but in smaller quantities.
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