Production of Exfoliative Toxin A by Staphylococcus aureus Isolated from Mastitic Cow’s Milk and Farm Bulk Milk

Journal of Veterinary Medical Science

Michiko HAYASHI²  Takeshi SHIMANO²  Nozomu HASHIMOTO³  Hideo IGARASHI¹  Kyuhei TAKEUCHI²  Shotaro TAKEUCHI³  Hirofumi KOMAE²  Miyoko ENDOU¹  Yuji HAYAKAWA² 

[1] Department of Microbiology, Tokyo Meteropolitan Research Laboratory of Public Health;Hokubu Livestock Hygiene Service Center;Department of Bioscience, Faculty of Biotechnology, Fukui Prefectural University

关键词: bovine mastitis;    exfoliative toxin A;    Staphylococcus aureus;   

DOI  :  10.1292/jvms.60.1281

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(19)Cited-By(8)The production of exfoliative toxins A and B (ETA and ETB) by Staphylococcus aureus isolated from mastitic cow's milk and farm bulk milk was examined by the reverse passive latex agglutination method (RPLA). ETA was detected in 2 (1.2%) of 162 isolates from mastitic cow's milk and in 1 (0.6%) of 166 isolates from farm bulk milk. RPLA titers of these isolates were much lower than in human isolates. No ETB was detected in any of the isolates tested. These ETA-positive isolates belonged to bovine ecovar. They were non-typable using the international phage set for human strains. When these ETA-positive isolates were subcutaneously inoculated into neonatal mice, general exfoliation of the epidermis accompanied by the so-called Nikolsky sign was not recognized. By the immunoblotting and PCR methods, however, ETA and eta gene were recognized in the ETA-positive isolates from mastitic cow's milk and farm bulk milk. These data suggest that ETA is also produced by bovine isolates of S. aureus, but in smaller quantities.

【 授权许可】

Unknown