Expression of Prolactin-Releasing Peptide in Human Placenta and Decidua

Endocrine Journal

TOSHIYA YAMAMOTO¹  YUJI MURATA¹  YUMIKO YASUI¹  MASAAKI YAMAGUCHI³  TORU KANZAKI¹  HIROAKI JIKIHARA² 

[1] Department of Specific Organ Regulation, Division of Obstetrics and Gynecology, Osaka University Graduate School of Medicine;Department of Obstetrics and Gynecology, Suita Municipal Hospital;Department of Gynecology, Kominami Memorial Hospital

关键词: PrRP;    PRL;    hGR3;    Placenta;    Human;   

DOI  :  10.1507/endocrj.48.397

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(15)Cited-By(2)The aims of this study were to determine whether the human placenta and decidua express PRL-releasing peptide (PrRP) mRNA and whether PrRP regulates PRL secretion from cultured human decidual cells. PrRP gene expression was analyzed by reverse transcription (RT)-PCR, and the level of the gene expression was quantified by a ribonuclease protection assay. PrRP gene expression was detected in both the placenta and decidua. These tissues expressed PrRP mRNA throughout pregnancy and the level of PrRP mRNA expression somewhat increased during midpregnancy. Placental and decidual cells also expressed PrRP mRNA, in vitro. To determine whether PrRP affects decidual PRL secretion, human endometrial stromal cells and decidual cells were cultured and treated with or without 1μM PrRP31. PrRP31 did not affect PRL secretion in either short or long term incubation. Moreover, the RT-PCR analysis indicated that human decidua does not express the PrRP receptor, hGR3, mRNA. These findings suggest that PrRP produced by the human placenta and decidua does not affect decidual PRL secretion due to a lack of the receptor, and that it may play other roles during pregnancy.

【 授权许可】

Unknown