Genetic effects in callose content in root apex of inbred resistant and susceptible corn lines to acid soils

Acta Agronómica

Caetano, Creuci¹  Narro, Luis Alberto²  Arcos, Alba Lucía²  Salazar, Fredy² 

[1] Universidad Nacional de Colombia, Palmira;Cimmyt, Cali-Palmira

关键词: Zea mays;    callose;    inheritance;    diallel;    heterosis;    acid soils;   

DOI  :  

学科分类：农业科学（综合）

来源: Universidad Nacional de Colombia * Facultad de Ciencias Agropecuarias Palmira

【 摘 要 】

The main objective of this work was to identify genetic parameters that are related to callose accumulation using tropical inbreeds corn. Fourteen inbreed lines selected for different levels of tolerance to acid soils were chosen and a diallel among them was generated. The seeds were grown under controlled environmental conditions in a growth chamber and transferred to a nutrient solution at pH 4.3. After 12 hours of 25 µM Al (AlCl3) treatment was applied, callose contents of 1 cm root apex were determined with fluorescence spectrophotometer. Significant differences were found for parents, crosses and parents vs crosses. Average callose content varied from 0.746 to 2.035 (µg PE/cm root apex). Heterosis varied from -21.2% to 276.3%. General combining ability (GCA) and specific combining ability (SCA) were highly significant indicating that both, additive and non-additive gene effects were important for callose inheritance. SCA and GCA sum of squares accounted for 54% and 46%, respectively of crosses of squares sum. Correlation coefficient between callose content and corn grain yield in the field was negative but not significant (r = -0.38).

【 授权许可】

CC BY-NC-SA