Inhibitory Effects of a Tryptamine Derivative on Ultraviolet Radiation–Induced Apoptosis in MC3T3-E1 Mouse Osteoblasts

Journal of Pharmacological Sciences

Masanori Somei⁴  Kuniyasu Ochiai³  Nobukazu Watanabe¹  Eri Watanabe¹  Motoki Senoo²  Kiyoshi Yamada³  Yoshikazu Mikami²  Mio Lee²  Minoru Takagi²  Masaki J. Honda² 

[1] Laboratory of Diagnostic Medicine, The Institute of Medical Science, The University of Tokyo, Japan;Department of Anatomy, Nihon University School of Dentistry, Japan;Department of Microbiology, Nihon University School of Dentistry, Japan;Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Japan

关键词: MS-IPA1;    tryptamine;    apoptosis;   

DOI  :  10.1254/jphs.10208FP

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(17)Cited-By(1)MS-IPA1 is a new synthetic compound that is synthesized from tryptamine. Recently, our group demonstrated that SST-VED-I-1, which has a similar chemical structure to MS-IPA1, inhibits starvation-induced apoptosis in osteoblasts. However, the effects of MS-IPA1 on apoptosis in osteoblasts have not yet been examined. Therefore, this study examined the effects of this compound on apoptosis in osteoblasts. In this study, MC3T3-E1 mouse osteoblasts were used and apoptosis was induced by ultraviolet radiation (UV). We investigated the effect of MS-IPA1 on apoptosis by analyzing caspase3/7 activity, translocation of phosphatidylserine (PS), and mRNA expression levels of Bcl-2 and Bax. In addition, it was investigated whether MS-IPA1 affects cell proliferation and cell cycle progression. We found that MS-IPA1 had no effect on cell proliferation or cell cycle progression. However, MS-IPA1 suppressed UV-induced cell death in a dose-dependent manner, which was accompanied with the inhibition of caspase activation and translocation of PS. Furthermore, after UV exposure, Bcl-2 expression was increased in the MS-IPA1–treated cells as compared to that in the vehicle-treated cells. In contrast, Bax expression was decreased in the MS-IPA1–treated cell as compared to that in the vehicle-treated cells. These results suggest that MS-IPA1 has an inhibitory effect on apoptosis in osteoblasts through a Bcl-2 family-dependent signaling pathway.

【 授权许可】

Unknown