Integrity of sperm plasm membrane, nucleus and mitochondria after freezing ram semen with ethylene glycol

Ciência Rural

Gonçalves, Paulo Bayard Dias²  Oliveira, João Francisco Coelho de³  Montagner, Marcelo Marcos³  Brisola, Luciana Bignardi de Soares¹  Neves, Jairo Pereira² 

[1] Universidade Federal de Santa Maria, Santa Maria;Universidade Federal de Santa Maria

关键词: semen;    ethylene glycol;    membrane integrity;    ram;   

DOI  :  10.1590/S0103-84781999000300025

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Ciencias Rurais

【 摘 要 】

The aim of the present experiment was to evaluate the effect of ethylene glycol, in relation to glycerol, as a cryoprotective agent for preserving ovine spermatic cells. The semen had to present a minimal quality to be used, regarding volume (0.5 ml), wave motion (score of 2, from 0 to 5), percentage of progressively motile spermatozoa (65%), rate of progressive motility (score of 3, from 0 to 5), sperm cell concentration (3x106 cells/mm3) and normal sperm morphology (80%). Each pool of semen from ejaculates of two rams was divided into two equal subsamples. One subsample was frozen with ethylene glycol and the other with glycerol. The parameters used to evaluate the semen were sperm motility, vigor, acrossome status and membrane integrity. Sperm motility was evaluated in fresh semen, cooled semen, frozen semen, after 5 hours at 38°C or after 30 minutes at 45°C. No difference was observed between ethylene glycol and glycerol for acrossome status and sperm motility. The sperm cells that were preserved with ethylene glycol showed more integrity of the plasmatic, nuclear and mitochondrial membranes. From the viewpoint of cell membrane integrity, it can be concluded that ethylene glycol gives higher protection to the sperm cell than glycerol.

【 授权许可】

CC BY-NC