Multivalent DNA Vaccine Enhanced Protection Efficacy against Infectious Bronchitis Virus in Chickens

Journal of Veterinary Medical Science

Hong-Ning WANG¹  Yu-Ling LI¹  Jun-Ni TANG¹  Juan LI¹  Tai YANG¹  Xue WANG¹  Rong GAO¹  Zi-Cheng GUO¹ 

[1] Animal Disease Prevention and Food Safety Key Lab of Sichuan Province, School of Life Sciences, Sichuan University

关键词: DNA vaccine;    infectious bronchitis virus;    multivalency;   

DOI  :  10.1292/jvms.001585

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(33)Cited-By(6)For efficacious DNA vaccine development against infectious bronchitis virus (IBV), the immunogenicity of a multivalent DNA vaccine was evaluated. Three expression plasmids each targeting spike protein (S1), nucleocapsid protein (N), and membrane protein (M) of IBV were prepared. Chickens were immunized with either individual plasmids (monovalent) or with a combination of all plasmids (multivalent). Immunization with the multivalent DNA vaccine induced synergistic augmentation of humoral and cellular responses in comparison with the individual vaccines, and provided up to 85% immune protection. Thus the multivalent DNA vaccine represents an innovative approach for enhancing DNA vaccine potency, and has potential clinical application for vaccination against IBV.

【 授权许可】

Unknown