Detection of hepatopancreatic parvovirus (HPV) in wild shrimp from India by nested polymerase chain reaction (PCR)

Diseases of Aquatic Organisms

K. R. Umesha¹  B. Manjanaik¹  Iddya Karunasagar¹  Indrani Karunasagar¹ 

关键词: Hepatopancreatic parvovirus;    HPV;    Penaeid shrimp;    PCR;    Captured wild shrimp;   

DOI  :  10.3354/dao063255

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: The prevalence of hepatopancreatic parvovirus (HPV) in wild penaeid shrimp samples from India was studied by nested polymerase chain reaction (PCR) using primers designed in our laboratory. The virus could be detected in 9 out of 119 samples bynon-nested PCR. However, by nested PCR 69 out of 119 samples were positive. The PCR results were confirmed by hybridization with digoxigenin-labelled DNA probe. Shrimp species positive by non-nested PCR included Penaeus monodon, Penaeus indicus andPenaeus semisulcatus and by nested PCR Parapenaeopsis stylifera, Penaeus japonicus, Metapenaeus monoceros, M. affinis, M. elegans, M. dobsoni, M. ensis and Solenocera choprai. This is the first report on the prevalence of HPV incaptured wild shrimp from India.

【 授权许可】

Unknown