Molecular Analysis of db Gene-related Pancreatic β Cell Dysfunction; Evidence for a Compensatory Mechanism Inhibiting Development of Diabetes in the db Gene Heterozygote

Endocrine Journal

Fumiko KAWASAKI¹  Sumiko HAMAMOTO¹  Fuminori TATSUMI¹  Kazuhito TAWARAMOTO¹  Koji NAKASHIMA¹  Kohei KAKU¹  Michihiro MATSUKI¹  Mitsuru HASHIRAMOTO¹  Yukiko KANDA¹  Masashi SHIMODA¹ 

[1] Diabetes and Endocrine Division, Department of Internal Medicine, Kawasaki Medical School, Kurashiki, Japan

关键词: db gene heterozygote;    β cell dysfunction;    Cell proliferation;    Cell apoptosis;    Oxidative stress;   

DOI  :  10.1507/endocrj.K09E-028

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(24)Cited-By(2)The db gene homozygous, but not heterozygous, mice develop diabetes with severe β-cell damage. We investigated the molecular mechanism underlying db gene-associated pancreatic β-cell dysfunction. Islet morphology, β-cell function, and gene expression profiles specific for pancreatic islet cells were compared among db gene homozygous(db/db), heterozygous (db/m) and unrelated m/m mice. The β-cell ratio decreased in db/db mice with age, but not in non-diabetic db/m and m/m mice. The islet insulin content was lower, but the triglyceride content was higher in db/db than other mice. The islet cell specific gene expression profiles analyzed by laser capture microdissection method and morphological findings suggested an augmentation of β-cell apoptosis, oxidative stress and ER stress in db/db mice. Interestingly, insulin I and II, anti-apoptotic bcl-2, and proliferation promoting ERK-1 gene expressions were significantly upregulated in db/m mice. An impaired glucose tolerance was shown in m/m mice fed a high fat diet, but not in db/m mice, in which a higher insulin response and increased β-cell mass were observed. Expressions of insulin I and II, bcl-2, and ERK-1 gene were increased in db/m mice, but not in m/m fed a high fat diet. The present results strongly suggest that the db gene heterozygote, but not homozygote, acquires a compensatory mechanism suppressing β-cell apoptosis and augmenting the capacity of β-cell function.

【 授权许可】

Unknown