Improved Electroporation of Rhodococcus equi

Journal of Veterinary Medical Science

Shiro TSUBAKI²  Shinji TAKAI²  Tsutomu SEKIZAKI¹  Makoto OSAKI¹  Yoshihiro SHIMOJI¹  Tomoaki TANOUE² 

[1] National Institute of Animal Health;Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University

关键词: electroporation;    genetic transformation;    Rhodococcus equi;   

DOI  :  10.1292/jvms.60.277

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(18)Cited-By(13)The condition of an electroporation method was re-evaluated for the introduction of foreign plasmid DNA into Rhodococcus equi. The method is based on an electroporation of the bacteria made competent by culturing in a broth containing glycine and by heat shock at 50°C. Transformation of R. equi could be achieved with a chloramphenicol-resistant shuttle vector originating from Rhodococcus fascians at an efficiency of about 104 transformants/ μg DNA. The bacteria were also shown to become competent when they were incubated with a chemical transformation buffer prior to washing with an electroporation buffer.

【 授权许可】

Unknown