Growth dynamics within bacterial communities in riverine and estuarine batch cultures

Aquatic Microbial Ecology

Eva S. Lindström¹  Silke Langenheder¹  Veljo Kisand¹  Johan Wikner¹  Lars J. Tranvik¹ 

关键词: Heterotrophic bacteria;    Growth dynamics;    Batch cultures;    DGGE;    DNA-DNA hybridization;   

DOI  :  10.3354/ame037137

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: We investigated temporal changes in community composition of bacteria growing on riverine dissolved organic carbon. Batch cultures were adjusted to riverine or estuarine salinity levels and inoculated with bacteria from these 2 environments totest whether growth patterns of bacterial taxa are influenced by salinity and/or the source of the inoculum. Changes in bacterial community composition at different stages of the growth phase were studied by 16S rDNA denaturing gradient gelelectrophoresis (DGGE). Furthermore, the growth dynamics of 7 bacteria previously isolated from the estuary were followed by quantitative DNA-DNA hybridization. Growth dynamics within bacterial communities were significantly influenced by the source ofthe inoculum but not by salinity, suggesting that slight changes in salinity, to which riverine bacteria are exposed when discharged into the Northern Baltic Sea, are not a major regulating factor of community dynamics. Additionally, our results indicatedonly minor differences in the appearance and growth of bacteria when examined by quantitative DNA-DNA hybridization, whereas DGGE banding patterns suggested that there were fast- and slow-growing types of bacteria.

【 授权许可】

Unknown