Histopathological alterations of the heart in fish: proposal for a standardized assessment

Diseases of Aquatic Organisms

Thomas Wahli¹  Hana Kocour Kroupová¹  Heike Schmidt-Posthaus¹  Jana Klicnarová¹  Christoph Steinbach¹ 

关键词: Cardiovascular system;    Functional unit;    Heart index;    Histopathology;    Ventricle;    Valves;    Bulbus arteriosus;   

DOI  :  10.3354/dao02971

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Histopathological alterations in the heart are often reported in fish as a result of exposure to a variety of chemical compounds. However, researchers presently lack a standardized method for the evaluation of histopathological alterations in the cardiovascular system of fish and the calculation of an ‘organ index�?. Therefore, we designed a method for a standardized assessment and evaluation of histopathological alterations in the heart of fish. As a model species, we used rainbow trout Oncorhynchus mykiss, but the protocol was also successfully applied to other fish species belonging to different taxonomic orders. To test the protocol, we re-evaluated sections of atenolol-exposed and unexposed rainbow trout obtained in a previous study. The results were in accordance with those previously published, demonstrating the applicability of the protocol. The protocol provides a universal method for the comparative evaluation of histopathological changes in the heart of fish.

【 授权许可】

Unknown