Susceptibility of zebra fish Danio rerio to infection by Flavobacterium columnare and F. johnsoniae

Diseases of Aquatic Organisms

David W. Hunnicutt¹  Thomas R. Moyer¹ 

关键词: Flavobacterium columnare;    Flavobacterium johnsoniae;    Zebra fish;   

DOI  :  10.3354/dao076039

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Flavobacterium columnare is a serious pathogen in a wide range of fish species. F. johnsoniae is an opportunistic pathogen of certain fish. Both are gliding bacteria. These species were tested for their ability to infect the zebra fish Danio rerio. Both injection and bath infection methods were tested. The results indicate that F. johnsoniae is not an effective pathogen in D. rerio, but that F. columnare is an effective pathogen. F. johnsoniae did not cause increased death rates following bath infection, but did cause increased death rates following injection, with an LD₅₀ (mean lethal dose) of approximately 3 × 10¹⁰ cfu (colony-forming units). Non-motile mutants of F. johnsoniae produced a similar LD₅₀. F. columnare caused increased death rates following both injection and bath infections. There was considerable strain variation in LD₅₀, with the most lethal strain tested producing an LD₅₀ of 3.2 × 10⁶ cfu injected and 1.1 × 10⁶ cfu ml^–1 in bath experiments, including skin damage. The LD₅₀ of F. columnare in zebra fish without skin damage was >1 × 10⁸, indicating an important effect of skin damage.

【 授权许可】

Unknown