Molecular typing of Salmonella enterica serovar 4,[5],12:i:-isolates from humans, animals and river water in Japan by multilocus variable-numbertandem repeat analysis and pulsed-field gel electrophoresis

Journal of Veterinary Medical Science

Gakuji YAEGASHI⁵  Masaru SUGAWARA⁶  Masaru KONNO²  Kiyoshi TANAKA⁴  Noriko IDO⁵  Yusuke SATO’O⁸  Ikuo UCHIDA⁷  Masato AKIBA¹  Kaori IWABUCHI³  Yasuo SATO⁵  Katsuhiko OMOE⁸ 

[1] National Institute of Animal Health, Tsukuba, Japan;Iwate Agricultural Research Center, Taneyama livestock laboratory, Oshu, Japan;Research Institute for Environmental Sciences and Public Health of Iwate Prefecture, Morioka, Japan;Hokkaido Research Station, National Institute of Animal Health, Sapporo, Japan;Iwate Prefecture Central Livestock Hygiene Service Center, Takizawa, Japan;Iwate Prefecture North Livestock Hygiene Service Center, Karumai, Japan;The United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan;Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Japan

关键词: MLVA;    monophasic variant;    PFGE;    Salmonella 4;    [5];    12:i:-(S. 4;    [5];    12:i:-);    S. Typhimurium;   

DOI  :  10.1292/jvms.14-0465

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(22)Fifty-one Salmonella enterica serovar 4,[5],12:i:-(S. 4, [5],12:i:-) isolates (14 human strains, 34 animal strains and 3river water strains) which are assumed to be monophasic variants of S.Typhimurium were analyzed using pulsed-field gel electrophoresis (PFGE) and multiple-locusvariable-number tandem repeat analysis (MLVA) in order to investigate their geneticdiversities and relationships. PFGE, MLVA and combination of them identified 28, 27 and 34profiles (Simpson’s diversity indices [DI]=0.94, 0.96 and 0.97), respectively. Nocorrelations were detected between MLVA clustering and PFGE clustering or phage typing.These results suggested that S. 4,[5],12:i:- originated from multipleS. Typhimurium ancestors. Two cattle and one pig isolates showingidentical phage types as well as PFGE and MLVA profiles to human isolatesS. 4,[5],12:i:- suggested the existence of the links between humaninfections and animal reservoirs.

【 授权许可】

Unknown