Replication of Virulent and Attenuated Strains of Aujeszky's Disease Virus in Swine Alveolar Macrophages

Journal of Veterinary Medical Science

Munenori OKADA¹  Katsuyoshi URUNO¹  Isao SHIBATA¹  Yasuo SAMEGAI¹  Yuji INABA¹ 

关键词: alveolar macrophage;    Aujeszky's disease virus;    in vitro replication;    in vivo replication;    swine;   

DOI  :  10.1292/jvms.56.465

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(16)Cited-By(4)In vitro and in vivo replication of Aujeszky's disease virus (ADV) in swine alveolar macrophages (AM) was studied using two virulent strains and a vaccine strain with deletions in the thymidine kinase and gIII genes. In vitro, AM were highly permissive to virulent ADV infection. Virus progeny titers of virulent strains in the cell phase and in the fluid phase were higher than 107.3 TCID50/ml at 84 hr post-inoculation (PI). For vaccine strain infection, AM were less permissive, yielding virus titers of 102.3 TCID50/ml at 84 hr PI. To study in vivo replication of ADV in AM, virus isolations were made from AM collected at intervals from pigs inoculated intranasally with both the virulent and vaccine strains. Virus was isolated from AM samples collected from all pigs infected with the virulent strain from days 2 to 22 PI. On the contrary, no virus was detected in AM samples collected from pigs infected with the vaccine strain. The results presented suggesited that in vivo virulent ADV replicates for a relatively long period in swine alveolar macrophages.

【 授权许可】

Unknown